| Expression pattern: |
UP |
| Associated gene: |
CENPF, Ago2 |
| Associated microRNA: |
miR-630 |
| Biological function: |
CircDLG1 promotes malignant progression of NSCLC by enhancing proliferation, migration, invasion, tumor growth, and angiogenesis-related marker expression, while inhibiting apoptosis. |
| Molecular mechanism: |
CircDLG1 acts as a sponge for miR-630 and positively regulates CENPF expression through a circDLG1/miR-630/CENPF ceRNA axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); angiogenesis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circDLG1 expression is associated with lymph node metastasis and lower overall survival in NSCLC patients, suggesting a connection with progression and poor prognosis. |
| Description: |
CircDLG1 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with lymph node metastasis and poor overall survival. Functionally, circDLG1 promotes NSCLC cell proliferation, migration, invasion, and in vivo tumor growth while suppressing apoptosis. Mechanistically, it acts as a miR-630 sponge to increase CENPF expression through the circDLG1/miR-630/CENPF axis. |
| Confidence score: |
0.7975 |