circRNA basic information
circBase ID: hsa_circ_0065173
Name: hsa_circ_SETD2
Synonym: circSETD2 / circular RNA circ SET domain containing 2 (circSETD2)
Host Gene: SETD2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

liver cancer tissues; para-cancerous tissues; transplanted tumor tissues
Cell lines:

HB611; SMMC-7721; HepG2; Hep3B; MHCC97L; L02
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: E-cadherin, Vimentin, N-cadherin, MMP-9, MMP-2
Associated microRNA: -
Biological function: Inhibits hepatocellular carcinoma cell proliferation, colony formation, invasion and migration; induces G0/G1 cell cycle arrest; suppresses tumor growth in vivo.
Molecular mechanism: Regulates EMT-associated proteins (increases E-cadherin; decreases N-cadherin and Vimentin) and invasion-related proteins (MMP-2, MMP-9); detailed upstream mechanism not determined.
Biological pathway or process:

proliferation (inhibits); cell cycle (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circSETD2 is down-regulated in hepatocellular carcinoma tissues and cell lines. Functional assays show circSETD2 acts as a tumor suppressor by inhibiting proliferation, colony formation, migration and invasion, inducing G0/G1 arrest, and reducing xenograft tumor growth, accompanied by changes in EMT/invasion-related proteins (E-cadherin, N-cadherin, Vimentin, MMP-2/9).
Confidence score:

0.6831
Other information
Title:

Circular RNA circ SET domain containing 2 (circSETD2) inhibits hepatocellular carcinoma cell proliferation and invasion in vivo and in vitro.

Journal: Bioengineered
Published: 2022
PubMed ID: 35260047
Study type:

combined biological and clinical study
Data availability: -
Code availability: -