circRNA basic information
circBase ID: hsa_circ_0004214
Name: hsa_circ_AMOTL1
Synonym: -
Host Gene: AMOTL1
Genomic location(hg19): chr11:94532555-94533477:+
Genomic location(hg38): chr11:94799389-94800311:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

cervical carcinoma specimens; paired normal peritumoral specimens
Cell lines:

Hela; SiHa; HcerEpic
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SIK2, Ago2, AKT, AMOTL1
Associated microRNA: miR-526b
Biological function: promotes proliferation and migration/invasion, inhibits apoptosis, promotes tumorigenicity and metastasis, and promotes EMT in cervical cancer
Molecular mechanism: ceRNA mechanism: circAMOTL1 sponges miR-526b to upregulate SIK2, activating AKT signaling and promoting EMT
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); PI3K/AKT (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

Upregulated circAMOTL1 was closely associated with tumor differentiation, FIGO staging, and HPV16/18 in cervical carcinomas.
Description:

CircAMOTL1 is up-regulated in cervical cancer tissues and cell lines and promotes proliferation, migration/invasion, EMT, tumorigenicity and metastasis while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-526b to increase SIK2 expression and enhance AKT signaling/EMT.
Confidence score:

0.7418
Other information
Title:

CircAMOTL1 Promotes Tumorigenesis Through miR-526b/SIK2 Axis in Cervical Cancer.

Journal: Frontiers in cell and developmental biology
Published: 2020
PubMed ID: 33344445
Study type:

combined biological and clinical study
Data availability: The original contributions presented in the study are included in the article/Supplementary Material.
Code availability: -