| Expression pattern: |
UP |
| Associated gene: |
VEGFA |
| Associated microRNA: |
miR-29a |
| Biological function: |
Promotes cardiac angiogenesis after MI by enhancing cardiac endothelial cell proliferation, migration, tube formation, and cell cycle progression. |
| Molecular mechanism: |
Exosomal circHIPK3 acts through a ceRNA-like mechanism to inhibit miR-29a activity, thereby increasing VEGFA expression and promoting angiogenic behaviors in cardiac endothelial cells. |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; EdU Staining; Transwell Assay; Tube Formation Assay; Cell Cycle Assay; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
This study shows that circHIPK3 is enriched in hypoxia-induced cardiomyocyte exosomes and increases in recipient cardiac endothelial cells. Exosomal circHIPK3 promotes post-MI angiogenesis by inhibiting miR-29a, upregulating VEGFA, and accelerating endothelial cell cycle progression, proliferation, migration, and tube formation. |
| Confidence score: |
0.6179 |