circRNA basic information
circBase ID: -
Name: hsa_circ_VRK1
Synonym: -
Host Gene: VRK1
Genomic location(hg19): chr14:97312431-97327072:.
Genomic location(hg38): chr14:96846094-96860735:.
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

MCF-7; MDA-MB-231
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: mir-153-5p
Biological function: Suppresses BCSC expansion and self-renewal; negatively regulates stemness of BCSCs.
Molecular mechanism: Predicted to act as a miRNA sponge (ceRNA), potentially adsorbing mir-153-5p to inhibit BCSC stemness.
Biological pathway or process:

stemness (inhibits); proliferation (inhibits)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; Transfection; Colony Formation Assay
Clinical significance:

-
Description:

circVRK1 is down-regulated in breast cancer stem cell-enriched mammospheres versus non-BCSCs. Functionally, circVRK1 suppresses BCSC self-renewal/expansion; its knockdown increases mammosphere/colony formation and stemness markers. Mechanistically, it is predicted to interact with mir-153-5p, suggesting a potential ceRNA-like inhibitory axis on stemness.
Confidence score:

0.6212
Other information
Title:

Circular RNA profile indicates circular RNA VRK1 is negatively related with breast cancer stem cells.

Journal: Oncotarget
Published: 2017
PubMed ID: 29221160
Study type:

combined biological and bioinformatics study
Data availability: -
Code availability: -