| Expression pattern: |
UP |
| Associated gene: |
ATG7, ATG7 mRNA, HUR, Argonaute 2 (AGO2) |
| Associated microRNA: |
miR-766-5p |
| Biological function: |
promotes proliferation; promotes mobility (migration/invasion); promotes metastasis; promotes autophagy; promotes EMT |
| Molecular mechanism: |
Cytoplasmic circATG7 sponges miR-766-5p to increase ATG7; nuclear circATG7 recruits HUR to enhance ATG7 mRNA stability and autophagy-dependent tumor progression. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; ISH (In Situ Hybridization); RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circATG7 expression was positively associated with tumor diameter and lymph node invasion in patients with PC; high expression of circATG7 was associated with a lower overall survival rate; ROC indicated diagnostic value to estimate PC and adjacent normal tissues. |
| Description: |
circATG7 is up-regulated in pancreatic cancer tissues and cell lines and is associated with more aggressive clinicopathologic features and poorer overall survival. Functionally, circATG7 promotes proliferation, migration/invasion, metastasis, EMT and autophagy via a dual mechanism: cytoplasmic sponging of miR-766-5p to increase ATG7 expression and nuclear recruitment of HUR to enhance ATG7 mRNA stability, leading to ATG7-dependent autophagic flux. |
| Confidence score: |
0.8901 |