circRNA basic information
circBase ID: hsa_circ_0027247
Name: hsa_circ_GLI1
Synonym: circ-GLI1
Host Gene: GLI1
Genomic location(hg19): chr12:57853917-57857874:+
Genomic location(hg38): chr12:57460134-57464091:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005105
MONDO name: melanoma
Disease details: melanoma
Disease DO ID:
 1909
Disease MeSH ID:
 D008545
Disease NCIt ID:
 C3224
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

xenografts
Cell lines:

HEMa-LP; A375; MEL-RM; B16; M14; SK-MEL-2; SK-MEL-28; HEK293T; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CYR61, p70S6K2, GSK3beta, GLI1, beta-catenin, MYC, RPS6KB2, ATP5F1A, HSPA5, RPL27, AHSG
Associated microRNA: -
Biological function: Promotes melanoma cell migration, invasion, metastasis and angiogenesis; upregulates CYR61 transcription.
Molecular mechanism: circ-GLI1 binds p70S6K2 (RPS6KB2), increases GSK3beta Ser9 phosphorylation, reduces GSK3beta binding to GLI1 and beta-catenin to prevent their degradation, activating Hedgehog/GLI1 and Wnt/beta-catenin signaling; induces MYC which transcriptionally activates CYR61.
Biological pathway or process:

Hedgehog (promotes); Wnt/beta-catenin (promotes); metastasis (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); EMT (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; divergent primers PCR; Sanger Sequencing; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Luciferase Reporter Assay; Transfection; Wound Healing Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; ChIP / ChIP-seq
Clinical significance:

-
Description:

circ-GLI1 (hsa_circ_0027247), derived from GLI1, is up-regulated in melanoma cells and promotes migration/invasion, metastasis, and angiogenesis. Mechanistically, it binds p70S6K2 to increase GSK3beta Ser9 phosphorylation, stabilizing GLI1 and beta-catenin proteins to activate Hedgehog/GLI1 and Wnt/beta-catenin signaling, thereby inducing MYC-driven CYR61 transcription.
Confidence score:

0.846
Other information
Title:

Circ-GLI1 promotes metastasis in melanoma through interacting with p70S6K2 to activate Hedgehog/GLI1 and Wnt/beta-catenin pathways and upregulate Cyr61.

Journal: Cell death & disease
Published: 2020
PubMed ID: 32732916
Study type:

combined biological and clinical study
Data availability: Supplementary Information accompanies this paper at (10.1038/s41419-020-02799-x).
Code availability: -