| Expression pattern: |
UP |
| Associated gene: |
GEF-H1, RhoA, E-cadherin |
| Associated microRNA: |
miR-133a |
| Biological function: |
promotes cell migration and metastasis |
| Molecular mechanism: |
Exosomal circ-133 derived from hypoxic cells is delivered into normoxic CRC cells and sponges miR-133a, relieving post-transcriptional repression of GEF-H1 and RhoA, activating RhoA and reducing E-cadherin membrane distribution to enhance migration/metastasis. |
| Biological pathway or process: |
migration (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; ELISA; In Vivo Animal Model; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
Exosomal circ-133 is increased with CRC progression and is expected to be a biomarker for monitoring tumor progression. |
| Description: |
In colorectal cancer, circ-133 is up-regulated and enriched in plasma exosomes, especially under hypoxia. Hypoxia-derived exosomal circ-133 is transferred to normoxic CRC cells and promotes migration/metastasis by sponging miR-133a to enhance the GEF-H1/RhoA axis and reduce E-cadherin membrane distribution. |
| Confidence score: |
0.7554 |