| Expression pattern: |
DN |
| Associated gene: |
Ago2, BCL2, MMP2, MMP9, cleaved caspase-3, cleaved caspase-9, BAX |
| Associated microRNA: |
hsa-miR-6768-5p |
| Biological function: |
promotes trophoblast cell migration and invasion; inhibits trophoblast cell apoptosis |
| Molecular mechanism: |
Acts as a miRNA sponge for hsa-miR-6768-5p (ceRNA mechanism), supported by luciferase reporter and AGO2-RIP; miR-6768-5p overexpression rescues hsa_circ_0000848 phenotypes. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential biomarker/therapeutic target implication for FGR (not formally tested with diagnostic/prognostic metrics). |
| Description: |
In FGR placental tissues, hsa_circ_0000848 is down-regulated. Functionally, hsa_circ_0000848 promotes trophoblast (HTR-8) migration/invasion and inhibits apoptosis by sponging hsa-miR-6768-5p, supported by luciferase and AGO2-RIP and rescue experiments. |
| Confidence score: |
0.8088 |