circRNA basic information
circBase ID: -
Name: hsa_circ_GCLM
Synonym: circRNA_100284
Host Gene: GCLM
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005070
MONDO name: neoplasm
Disease details: arsenite-induced malignant transformation
Disease DO ID:
 14566
Disease MeSH ID:
 D009369
Disease NCIt ID:
 C3262
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

sera
Cell lines:

L-02; As-L-02-p20
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2, cyclin-D1
Associated microRNA: miR-217
Biological function: accelerates the cell cycle; promotes proliferation; promotes malignant transformation; promotes migration and invasion
Molecular mechanism: Exosomal circRNA_100284 acts as a sponge of miR-217, thereby regulating EZH2 and cyclin-D1 to accelerate G1/S transition and promote proliferation, contributing to arsenite-induced malignant transformation.
Biological pathway or process:

cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Western Blot; Transfection; Cell Cycle Assay; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

exosomal circRNA_100284 was upregulated in the sera of people exposed to arsenite and may be a biomarker for arsenite exposure/arsenicosis
Description:

circRNA_100284 (from host gene GCLM) is upregulated during arsenite-induced malignant transformation of human hepatic epithelial cells and is enriched in exosomes released by transformed cells. Exosomal circRNA_100284 is transferred to neighboring non-transformed cells, where it sponges miR-217, leading to increased EZH2/cyclin-D1, accelerated G1/S transition, enhanced proliferation, and promotion of malignant phenotypes. Circulating exosomal circRNA_100284 is increased in sera of arsenite-exposed individuals, suggesting biomarker potential for arsenite exposure/arsenicosis.
Confidence score:

0.5758
Other information
Title:

Exosomal circRNA_100284 from arsenite-transformed cells, via microRNA-217 regulation of EZH2, is involved in the malignant transformation of human hepatic cells by accelerating the cell cycle and promoting cell proliferation.

Journal: Cell death & disease
Published: 2018
PubMed ID: 29674685
Study type:

combined biological and clinical study
Data availability: Supplementary Information accompanies this paper at 10.1038/s41419-018-0485-1.
Code availability: -