| Expression pattern: |
UP |
| Associated gene: |
HMGA2, Twist1, Ago2 |
| Associated microRNA: |
miR-370 |
| Biological function: |
promotes proliferation, migration, invasion, and tumor growth in lung adenocarcinoma |
| Molecular mechanism: |
circASPH is transcriptionally upregulated by HMGA2/Twist1 and functions as a ceRNA sponge for miR-370 to relieve repression of HMGA2, forming an HMGA2-circASPH-HMGA2 positive feedback axis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry); ChIP / ChIP-seq; Co-IP; Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
circASPH level was positively correlated with T and N stages; higher circASPH predicted shorter overall survival |
| Description: |
circASPH is up-regulated in lung adenocarcinoma tissues and cell lines and is associated with more advanced T/N stages and shorter overall survival. It promotes proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it is transcriptionally regulated by HMGA2 and Twist1 and sponges miR-370 to relieve repression of HMGA2, forming an HMGA2-circASPH-HMGA2 positive feedback axis. |
| Confidence score: |
0.8775 |