| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-498 |
| Biological function: |
Promotes NSCLC cell proliferation, migration and invasion; associated with advanced TNM stage, lymph node metastasis, poor differentiation and shorter overall survival. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-498; circFADS2 inhibition increases miR-498 and miR-498 inhibitors rescue the phenotypes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; RNase R Treatment; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Wound Healing Assay; Transwell Assay |
| Clinical significance: |
High expression of circFADS2 was associated with advanced TNM stage, lymph node metastasis, poor differentiation, and shorter overall survival in NSCLC patients. |
| Description: |
circFADS2 (hsa_circRNA_100833, host gene FADS2) is up-regulated in NSCLC tissues and cell lines and promotes proliferation, migration and invasion. Mechanistically, it sponges miR-498 as supported by binding-site prediction, luciferase assays and rescue experiments with miR-498 inhibitors, and its high expression is associated with shorter overall survival. |
| Confidence score: |
0.814 |