| Expression pattern: |
UP |
| Associated gene: |
SLC7A11 / Slc7a11 / xCT, AGO2 |
| Associated microRNA: |
miR-1184 |
| Biological function: |
promotes tumor growth and protects LUAD cells from erastin-induced ferroptosis by promoting GSH synthesis |
| Molecular mechanism: |
ceRNA mechanism: circP4HB sponges miR-1184 to relieve repression of SLC7A11, enhancing SLC7A11-mediated GSH synthesis and suppressing ferroptosis |
| Biological pathway or process: |
ferroptosis (inhibits); glutaminolysis (not specified); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; IF (Immunofluorescence); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
identified as a novel biomarker in LUAD |
| Description: |
circP4HB (hsa_circ_0046263), derived from P4HB, is up-regulated in LUAD and functions as a ferroptosis suppressor. It acts as a ceRNA by sponging miR-1184 to increase SLC7A11 (xCT), promoting glutathione synthesis, reducing lipid peroxidation/ROS, and thereby protecting LUAD cells from erastin-induced ferroptosis and promoting tumor growth in xenografts. |
| Confidence score: |
0.7876 |