circRNA basic information
circBase ID: hsa_circ_0001649
Name: hsa_circ_SHPRH
Synonym: -
Host Gene: SHPRH
Genomic location(hg19): chr6:146209155-146216113:-
Genomic location(hg38): chr6:145888019-145894977:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005133
MONDO name: endometriosis
Disease details: Endometriosis
Disease DO ID:
 289
Disease MeSH ID:
 D004715
Disease NCIt ID:
 C3014
Disease ICD11 ID:
 1838213761
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ectopic endometrium samples; eutopic endometrium samples; normal endometrial samples
Cell lines:

ThESCs; ESCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: MMP9, estrogen receptors (ERs), E2
Associated microRNA: -
Biological function: Downregulation of hsa_circ_0001649 promotes stromal cell migration and invasion and contributes to E2-enhanced invasiveness via increasing MMP9.
Molecular mechanism: E2 downregulates hsa_circ_0001649 via estrogen receptors (ERs); decreased hsa_circ_0001649 is involved in E2-mediated upregulation of MMP9, promoting stromal cell invasion/migration.
Biological pathway or process:

migration (inhibits); invasion (inhibits); metastasis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; Western Blot; Transwell Assay; Clinical Sample Validation
Clinical significance:

-
Description:

In endometriosis, hsa_circ_0001649 is decreased in eutopic and ectopic endometrium and is downregulated by estradiol via estrogen receptors. Reduced hsa_circ_0001649 contributes to E2-driven MMP9 upregulation and promotes stromal cell migration and invasion, while circRNA overexpression reverses these effects.
Confidence score:

0.6672
Other information
Title:

Estrogen-decreased hsa_circ_0001649 promotes stromal cell invasion in endometriosis.

Journal: Reproduction (Cambridge, England)
Published: 2020
PubMed ID: 32698139
Study type:

combined biological and clinical study
Data availability: -
Code availability: -