circRNA basic information
circBase ID: hsa_circ_0005397
Name: hsa_circ_RHOT1
Synonym: circRHOT1
Host Gene: RHOT1
Genomic location(hg19): chr17:30500849-30503232:+
Genomic location(hg38): chr17:32173830-32176213:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009831
MONDO name: malignant pancreatic neoplasm
Disease details: pancreatic cancer
Disease DO ID:
 1793
Disease MeSH ID:
 -
Disease NCIt ID:
 C9005
Disease ICD11 ID:
 542147910
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PDAC fresh-frozen tissues; adjacent non-tumour tissues
Cell lines:

PANC-1; SW1990; COLO357; CF-PAC1; HPDE; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F3
Associated microRNA: miR-125a-3p
Biological function: promotes proliferation and invasion and inhibits apoptosis of pancreatic cancer cells
Molecular mechanism: circRHOT1 acts as an endogenous sponge of miR-125a-3p to regulate E2F3 expression
Biological pathway or process:

proliferation (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; Western Blot
Clinical significance:

circRHOT1 expression level was correlated with lymphatic metastasis in PDAC; may serve as a potential diagnostic marker and therapeutic target
Description:

circRHOT1 is up-regulated in PDAC tissues and pancreatic cancer cell lines. It promotes proliferation and invasion and suppresses apoptosis by sponging miR-125a-3p, thereby increasing E2F3 expression.
Confidence score:

0.6973
Other information
Title:

CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR-125a-3p.

Journal: Journal of cellular and molecular medicine
Published: 2020
PubMed ID: 32697386
Study type:

combined biological and clinical study
Data availability: All data, models or code generated or used during the study are available from the corresponding author by request.
Code availability: All data, models or code generated or used during the study are available from the corresponding author by request.