circRNA basic information
circBase ID: hsa_circ_0000326
Name: hsa_circ_MALAT1
Synonym: -
Host Gene: MALAT1
Genomic location(hg19): chr11:65238307-65246429:.
Genomic location(hg38): chr11:65470836-65478958:.
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

exosomes; prostate cancer cells
Cell lines:

LNCaP
In vivo animal model:

genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: AR, MALAT1, QKI, YY1
Associated microRNA: miR-338-3p
Biological function: promotes proliferation, migration, invasion, EMT and metastasis; upregulates AR expression
Molecular mechanism: exosomal circRNA-mediated ceRNA mechanism; YY1 enhances MALAT1 transcription via super-enhancer and upregulates QKI to promote circRNA biogenesis
Biological pathway or process:

migration (promotes); invasion (promotes); proliferation (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transwell Assay; Wound Healing Assay; CCK8; Colony Formation Assay; In Vivo Animal Model; H&E Staining; Western Blot; ChIP / ChIP-seq; Co-IP
Clinical significance:

Exosomal hsa-circ-0000326 may be a potential therapeutic target for metastatic PCa.
Description:

YY1-overexpressing M2 macrophages increase secretion of exosomes containing hsa-circ-0000326, which is transferred into prostate cancer cells and promotes migration, invasion, proliferation, EMT, and metastasis. Mechanistically, hsa-circ-0000326 acts as a miR-338-3p sponge to increase AR expression, while YY1 also promotes its biogenesis by enhancing MALAT1 transcription through a super-enhancer and upregulating QKI.
Confidence score:

0.8027
Other information
Title:

Upregulation of YY1 in M2 macrophages promotes secretion of exosomes containing hsa-circ-0000326 via super-enhancers to facilitate prostate cancer progression.

Journal: Molecular and cellular biochemistry
Published: 2025
PubMed ID: 39960585
Study type:

combined biological and clinical study
Data availability: -
Code availability: -