| Expression pattern: |
UN |
| Associated gene: |
PDK1, AKT1 |
| Associated microRNA: |
- |
| Biological function: |
Enhances cell proliferation and survival, decreases apoptosis, and protects against Dox-induced cardiomyopathy (cardiac repair). |
| Molecular mechanism: |
circ-Amotl1 physically binds PDK1 and AKT1, facilitating AKT phosphorylation and nuclear translocation of pAKT (and related proteins), forming RNase-sensitive ternary complexes. |
| Biological pathway or process: |
PI3K/AKT (promotes); apoptosis (inhibits); proliferation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
B
H
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Northern Blot; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; H&E Staining; TUNEL |
| Clinical significance: |
Potential therapeutic approach for preventing adverse cardiac remodeling in doxorubicin-induced cardiomyopathy. |
| Description: |
circ-Amotl1 physically binds PDK1 and AKT1 to promote AKT phosphorylation and nuclear translocation of pAKT, thereby enhancing cell proliferation/survival and reducing apoptosis. In vivo delivery of circ-Amotl1 alleviates doxorubicin-induced cardiomyopathy and adverse remodeling in mice, supporting a cardioprotective/therapeutic role. |
| Confidence score: |
0.7451 |