| Expression pattern: |
UP |
| Associated gene: |
p70S6K1 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Promotes breast cancer cell proliferation/growth, migration, and invasion; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-145-5p, thereby upregulating p70S6K1. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circZNF609 expression was closely associated with poor outcome/poor overall survival in breast cancer patients. |
| Description: |
circZNF609 is up-regulated in breast cancer and promotes proliferation, migration, invasion, and xenograft tumor growth. It functions as a ceRNA by sponging miR-145-5p, which increases p70S6K1 expression; high circZNF609 is associated with poor overall survival. |
| Confidence score: |
0.8202 |