| Expression pattern: |
DN |
| Associated gene: |
ZFP36, Ago2 |
| Associated microRNA: |
miR-182 / hsa-miR-182-5p |
| Biological function: |
Suppresses epithelial-mesenchymal transition, cell invasion, migration, cell cycle progression, and tumor growth; promotes apoptosis and autophagy in breast cancer cells. |
| Molecular mechanism: |
circRNA_000554 acts as a ceRNA sponge for miR-182, thereby relieving miR-182-mediated repression of ZFP36 and blocking EMT in breast cancer. |
| Biological pathway or process: |
EMT (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); autophagy (promotes); cell cycle (inhibits); ceRNA regulation (other); proliferation (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circRNA_000554 expression was associated with better prognosis and survival in breast cancer patients. |
| Description: |
circRNA_000554 is down-regulated in breast cancer tissues and cell lines, and higher expression is associated with better patient survival. Functionally, circRNA_000554 suppresses EMT, migration, invasion, and xenograft tumor growth while promoting apoptosis and autophagy. Mechanistically, cytoplasmic circRNA_000554 sponges miR-182 to upregulate ZFP36, forming a circRNA_000554/miR-182/ZFP36 tumor-suppressive axis. |
| Confidence score: |
0.8769 |