circRNA basic information
circBase ID: hsa_circ_0006877
Name: hsa_circ_LDLR
Synonym: circLDLR
Host Gene: LDLR
Genomic location(hg19): chr19:11230767-11231198:+
Genomic location(hg38): chr19:11120091-11120522:+
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008487
MONDO name: polycystic ovary syndrome
Disease details: polycystic ovary syndrome
Disease DO ID:
 11612
Disease MeSH ID:
 D011085
Disease NCIt ID:
 C26862
Disease ICD11 ID:
 1213633323
Disease OMIM ID:
 184700
Species: Human
Species details: Homo sapiens
Tissue specimen:

follicular fluid exosomes; granulosa cells
Cell lines:

KGN
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: CYP19A1, BMP6, INSR, AKRIC2, ADCY7, FZD4, PTPN11, BZRAP1
Associated microRNA: miR-1294, hsa-miR-1827, hsa-miR-326, hsa-miR-330-5p, hsa-miR-486-3p, hsa-miR-649
Biological function: Regulates estradiol (E2) secretion in recipient granulosa cells by modulating miR-1294 and CYP19A1; depletion inhibits E2 production.
Molecular mechanism: ceRNA mechanism: circLDLR sponges miR-1294 to derepress CYP19A1, affecting aromatase-dependent estradiol production; exosomal transfer mediates intercellular regulation.
Biological pathway or process:

ovarian steroidogenesis (other); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

circRNA-seq; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Sanger Sequencing; Luciferase Reporter Assay; Transfection; Western Blot; ELISA; Bioinformatics Analysis
Clinical significance:

-
Description:

Exosomal circLDLR (hsa_circ_0006877) is decreased in follicular fluid exosomes from PCOS patients. It acts as a ceRNA by sponging miR-1294, thereby relieving repression of CYP19A1 and modulating aromatase-dependent estradiol (E2) production in granulosa cells.
Confidence score:

0.7684
Other information
Title:

Depletion of exosomal circLDLR in follicle fluid derepresses miR-1294 function and inhibits estradiol production via CYP19A1 in polycystic ovary syndrome.

Journal: Aging
Published: 2020
PubMed ID: 32651991
Study type:

combined biological and clinical study
Data availability: GSE145461
Code availability: -