circRNA basic information
circBase ID: hsa_circ_0048676
Name: hsa_circ_UHRF1
Synonym: Circular ubiquitin-like, containing PHD and ring finger domains 1
Host Gene: UHRF1
Genomic location(hg19): chr19:4930727-4951008:+
Genomic location(hg38): chr19:4930715-4950996:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumor tissue samples; adjacent tissue samples; lung tissues
Cell lines:

HL-7702; SNU-182; Huh-7; HepG2; HuH-6; Bel-100; Bel-7402; Bel-7405; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EIF4A3, G9a mRNA, UHRF1 mRNA, G9a, UHRF1, PDLIM1
Associated microRNA: -
Biological function: Promotes HCC cell proliferation, colony formation, invasion, migration, EMT, tumorigenesis, progression, and pulmonary metastasis.
Molecular mechanism: circUHRF1 recruits EIF4A3 to increase the stability of G9a and UHRF1 mRNAs; G9a and UHRF1 cooperatively inhibit PDLIM1 transcription, resulting in PDLIM1 inhibition and activation of the Hippo-YAP pathway.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); Hippo/YAP (promotes); mRNA stability (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; IF (Immunofluorescence); IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circUHRF1 expression was correlated with poor HCC prognosis, tumor size, microvascular invasion, and independent risk for HCC development.
Description:

circUHRF1 is up-regulated in human HCC tissues and cell lines, and high expression is associated with poor prognosis. Functionally, circUHRF1 promotes HCC proliferation, migration, invasion, EMT, and metastasis. Mechanistically, it recruits EIF4A3 to stabilize G9a and UHRF1 mRNAs, leading to PDLIM1 transcriptional inhibition and Hippo-YAP pathway activation.
Confidence score:

0.9014
Other information
Title:

Circ ubiquitin-like-containing plant homeodomain and RING finger domains protein 1 increases the stability of G9a and ubiquitin-like-containing plant homeodomain and RING finger domains protein 1 messenger RNA through recruiting eukaryotic translation initiation factor 4A3, transcriptionally inhibiting PDZ and homeobox protein domain protein 1, and promotes the metastasis of hepatocellular carcinoma.

Journal: Journal of gastroenterology and hepatology
Published: 2024
PubMed ID: 38059880
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -