| Expression pattern: |
UP |
| Associated gene: |
GOT2 |
| Associated microRNA: |
miR-520a-5p |
| Biological function: |
promotes NSCLC cell proliferation, migration, invasion, and malate-aspartate metabolism |
| Molecular mechanism: |
circ-SEC31A acts as a miRNA sponge for miR-520a-5p to upregulate GOT2, thereby enhancing malate-aspartate metabolism |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); other pathway/process (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
High circ-SEC31A expression predicted a poor prognosis and served as an independent risk factor for the post-surgical overall survival of NSCLC patients |
| Description: |
circ-SEC31A (hsa_circ_0001421) is upregulated in NSCLC and promotes proliferation, migration, invasion and malate-aspartate metabolism. It functions as a ceRNA by sponging miR-520a-5p to increase GOT2 expression, and high circ-SEC31A indicates poor overall survival. |
| Confidence score: |
0.8237 |