circRNA basic information
circBase ID: hsa_circ_0000745
Name: hsa_circ_SPECC1
Synonym: circ‐SPECC1
Host Gene: SPECC1
Genomic location(hg19): chr17:20107645-20109225:+
Genomic location(hg38): chr17:20204332-20205912:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor
Cell lines:

HepG2; Huh‐7
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: TGFbeta2, Beclin1, p62
Associated microRNA: miR‐33a
Biological function: promotes proliferation and suppresses apoptosis to promote HCC tumorigenesis under oxidative stress; promotes tumor growth in vivo
Molecular mechanism: Acts as a miRNA sponge for miR-33a, thereby regulating TGFbeta2 and autophagy under oxidative stress.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); autophagy (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; TUNEL; Western Blot; Bioinformatics Analysis; In Vivo Animal Model
Clinical significance:

-
Description:

In H2O2-induced oxidative stress conditions, circ-SPECC1 is down-regulated in HCC cells but functions pro-tumorigenically. It promotes proliferation and suppresses apoptosis by sponging miR-33a, thereby relieving miR-33a-mediated repression of TGFbeta2 and modulating autophagy, and its knockdown reduces xenograft tumor growth.
Confidence score:

0.5627
Other information
Title:

Circ-SPECC1 modulates TGFbeta2 and autophagy under oxidative stress by sponging miR-33a to promote hepatocellular carcinoma tumorigenesis.

Journal: Cancer medicine
Published: 2020
PubMed ID: 32627938
Study type:

biological research
Data availability: The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.
Code availability: -