circRNA basic information
circBase ID: hsa_circ_0045942
Name: hsa_circ_TIMP2
Synonym: circTIMP2
Host Gene: TIMP2
Genomic location(hg19): chr17:76849058-76867088:-
Genomic location(hg38): chr17:78852976-78871006:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0011385
MONDO name: intervertebral disk degenerative disorder
Disease details: intervertebral disc degeneration
Disease DO ID:
 90
Disease MeSH ID:
 D055959
Disease NCIt ID:
 C26983
Disease ICD11 ID:
 -
Disease OMIM ID:
 603932
Species: Human
Species details: Homo sapiens
Tissue specimen:

degenerative nucleus pulposus (NP) tissues
Cell lines:

-
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MMP2, AGO2
Associated microRNA: miR-185-5p
Biological function: promoted ECM catabolism and suppressed ECM anabolism in NP cells, contributing to IDD progression
Molecular mechanism: Acts as a competitive endogenous RNA (ceRNA) by sponging miR-185-5p to upregulate MMP2, driving ECM degradation
Biological pathway or process:

ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; Northern Blot; Clinical Sample Validation
Clinical significance:

Higher circ-TIMP2 expression in IDD NP tissues and positively correlated with IDD grade
Description:

circ-TIMP2 is up-regulated in degenerative NP tissues and correlates with IDD grade. It acts in the cytoplasm as a ceRNA by sponging miR-185-5p, thereby relieving repression of MMP2 and promoting ECM catabolism while suppressing ECM anabolism in NP cells.
Confidence score:

0.8006
Other information
Title:

Circular RNA derived from TIMP2 functions as a competitive endogenous RNA and regulates intervertebral disc degeneration by targeting miR‑185‑5p and matrix metalloproteinase 2.

Journal: International journal of molecular medicine
Published: 2020
PubMed ID: 32626912
Study type:

combined biological and clinical study
Data availability: The data generated or analyzed during the present study are included in this published article with the exception of the siRNA sequences, which are patented.
Code availability: -