| Expression pattern: |
UP |
| Associated gene: |
STAT3, p300, NF-kappaB |
| Associated microRNA: |
- |
| Biological function: |
promotes glioma cell proliferation and invasion/metastasis under macrophage co-culture; promotes macrophage M2 polarization via exosomal transfer |
| Molecular mechanism: |
STAT3 transcriptionally upregulates circ-001422; exosomal circ-001422 binds p300 and STAT3 to enhance STAT3 K685 acetylation and activate STAT3/NF-kappaB nuclear signaling, promoting M2 polarization |
| Biological pathway or process: |
NF-kappaB (promotes); immune regulation (promotes); macrophage polarization (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Survival Analysis; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
A poorer prognosis was associated with elevated levels of circ-001422 in glioma tissues. |
| Description: |
circ-001422 is up-regulated in glioma tissues/cells and predicts poor overall survival. Glioma cells package circ-001422 into exosomes and transfer it to macrophages, where it binds p300 and STAT3 to increase STAT3 acetylation and activate STAT3/NF-kappaB signaling, promoting M2 polarization and enhancing glioma proliferation and invasion/metastasis. |
| Confidence score: |
0.834 |