circRNA basic information
circBase ID: hsa_circ_0008574
Name: hsa_circ_ANRIL
Synonym: circ_ANRIL
Host Gene: ANRIL
Genomic location(hg19): chr9:22046749-22056386:+
Genomic location(hg38): chr9:22046750-22056387:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005203
MONDO name: ischemia reperfusion injury
Disease details: cerebral ischemia-reperfusion
Disease DO ID:
 -
Disease MeSH ID:
 D015427
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HBMECs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: NF-kappaB pathway
Associated microRNA: miR-622
Biological function: Promotes OGD/R-induced cell injury by inhibiting proliferation and increasing apoptosis and inflammatory cytokine release in HBMECs; circ_ANRIL silencing is protective.
Molecular mechanism: circ_ANRIL functions as a ceRNA by sponging miR-622, thereby modulating NF-kappaB pathway activation in OGD/R-treated HBMECs.
Biological pathway or process:

NF-kappaB (promotes); apoptosis (promotes); inflammation (promotes); proliferation (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA
Clinical significance:

-
Description:

circ_ANRIL is up-regulated in an in vitro cerebral ischemia-reperfusion injury model (OGD/R-treated HBMECs) and exacerbates cell injury by suppressing proliferation while promoting apoptosis and inflammatory cytokine secretion. Mechanistically, circ_ANRIL sponges miR-622 and thereby promotes NF-kappaB pathway activation; circ_ANRIL knockdown is protective and this protection is reversed by miR-622 inhibition.
Confidence score:

0.5607
Other information
Title:

Silencing of circular RNA ANRIL attenuates oxygen-glucose deprivation and reoxygenation-induced injury in human brain microvascular endothelial cells by sponging miR-622.

Journal: Biological research
Published: 2020
PubMed ID: 32616043
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in the published article.
Code availability: -