| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-383-5p |
| Biological function: |
enhances CRC cell proliferation, invasion, and migration, while inhibiting apoptosis; knockdown suppresses proliferation, migration, and invasion and promotes apoptosis |
| Molecular mechanism: |
hsa_circ_0077007 sponges miR-383-5p and inhibits miR-383-5p expression; miR-383-5p target genes were enriched in CRC-related pathways including p53 signaling pathway and Colorectal cancer pathway |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); p53 signaling (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Clinical Sample Validation; Bioinformatics Analysis; ROC Analysis; Survival Analysis; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; RNA Pull-Down; Luciferase Reporter Assay |
| Clinical significance: |
high serum expression is associated with tumor size, N stage, M stage, TNM stage, diagnostic potential for distinguishing CRC patients from healthy individuals, and unfavorable overall survival |
| Description: |
hsa_circ_0077007 is up-regulated in CRC serum and CRC cell lines and may serve as a diagnostic and prognostic biomarker, with high expression indicating poorer overall survival. Functionally, hsa_circ_0077007 promotes CRC cell proliferation, migration, and invasion and suppresses apoptosis. Mechanistically, it binds/sponges miR-383-5p, with downstream miR-383-5p targets enriched in CRC-related pathways including p53 signaling. |
| Confidence score: |
0.7942 |