| Expression pattern: |
UP |
| Associated gene: |
COL1A1 |
| Associated microRNA: |
miR-193a-5p / hsa-miR-193a-5p |
| Biological function: |
promotes migration and invasion; promotes EMT; promotes liver metastasis |
| Molecular mechanism: |
ceRNA mechanism: circCSPP1 sponges miR-193a-5p to upregulate COL1A1, promoting EMT and metastasis |
| Biological pathway or process: |
EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; RNA-seq |
| Clinical significance: |
upregulation was correlated with a higher M stage; high circCSPP1 expression was associated with lower overall survival; circCSPP1 was an independent prognostic factor for CRC patients |
| Description: |
circCSPP1 (hsa_circ_0001806) is up-regulated in colorectal carcinoma and promotes EMT, migration/invasion, and liver metastasis. It acts as a ceRNA by sponging miR-193a-5p to relieve repression of COL1A1, increasing COL1A1 expression and EMT marker changes. High circCSPP1 is associated with higher M stage and worse overall survival in the patient cohort. |
| Confidence score: |
0.8734 |