| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, FOXM1 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation, migration, and in vivo tumor growth in cervical cancer. |
| Molecular mechanism: |
m6A-modified circARHGAP12 binds the m6A reader IGF2BP2 and facilitates IGF2BP2 association with FOXM1 mRNA, increasing FOXM1 mRNA stability and FOXM1 protein level. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); mRNA stability (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High circARHGAP12 level is associated with lower patient survival rate in cervical cancer. |
| Description: |
circARHGAP12 (hsa_circ_0000231), derived from ARHGAP12, is up-regulated in cervical cancer and predicts worse survival. It promotes cervical cancer proliferation, migration, and xenograft growth by binding the m6A reader IGF2BP2 and enhancing FOXM1 mRNA stability (circARHGAP12/IGF2BP2/FOXM1 complex). |
| Confidence score: |
0.8791 |