circRNA basic information
circBase ID: -
Name: hsa_circ_GFRA1
Synonym: hsa_circ_005239
Host Gene: GFRA1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

TNBC tissues; paired adjacent normal mammal tissues
Cell lines:

MDA-MB-231; BT549; MDA-MB-468; BT-20
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: GFRA1
Associated microRNA: miR-34a
Biological function: promotes proliferation; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-34a to regulate GFRA1; circGFRA1 and GFRA1 regulate each other by competing for miR-34a.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

high circGFRA1 expression level was significantly associated with poor TNBC outcomes; may be a diagnostic biomarker and potential target for TNBC therapy
Description:

circGFRA1 (hsa_circ_005239), derived from GFRA1, is up-regulated in TNBC and mainly localized in the cytoplasm. It promotes TNBC cell proliferation and inhibits apoptosis, and enhances tumor growth in xenografts. Mechanistically, circGFRA1 acts as a ceRNA by sponging miR-34a to derepress GFRA1, and high circGFRA1 is associated with poorer OS/DFS.
Confidence score:

0.8069
Other information
Title:

circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2017
PubMed ID: 29037220
Study type:

combined biological and clinical study
Data availability: -
Code availability: -