| Expression pattern: |
UP |
| Associated gene: |
HuR, NUCKS1, hnRNPA2B1 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, invasion, migration, tumor growth and metastasis; promotes extracellular vesicles-mediated macrophage M2 polarization |
| Molecular mechanism: |
forms an RNA-protein complex with HuR to increase NUCKS1 expression; activates PI3K/AKT/mTOR signaling; hnRNPA2B1 mediates packaging of circATP9A into extracellular vesicles that induce TAM M2 polarization |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); macrophage polarization (promotes); immune regulation (other); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; Gel Electrophoresis; ISH (In Situ Hybridization); FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis |
| Clinical significance: |
circATP9A is upregulated in NSCLC tissues and higher in advanced tumor stages; proposed as a diagnostic indicator and therapeutic target |
| Description: |
circATP9A is up-regulated in NSCLC and promotes proliferation, migration/invasion, tumor growth and metastasis. Mechanistically, it binds the RBP HuR to increase NUCKS1 mRNA/protein and activate PI3K/AKT/mTOR signaling, and it is packaged into extracellular vesicles via hnRNPA2B1 to drive TAM M2 polarization, promoting NSCLC progression. |
| Confidence score: |
0.8446 |