| Expression pattern: |
UP |
| Associated gene: |
SRSF10, Ago2, MMP2, VEGFA |
| Associated microRNA: |
miR-526b-3p |
| Biological function: |
promotes angiogenesis (cell viability, migration and tube formation) of glioma-associated endothelial cells |
| Molecular mechanism: |
SRSF10 promotes circ-ATXN1 biogenesis; circ-ATXN1 acts as a ceRNA sponge binding miR-526b-3p in Ago2/RISC to relieve miR-526b-3p-mediated repression of MMP2 and VEGFA |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); migration (promotes); invasion (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ-ATXN1 is up-regulated in glioma-associated endothelial cells and promotes angiogenesis-related behaviors (viability, migration, tube formation). Mechanistically, SRSF10 promotes circ-ATXN1 biogenesis, and circ-ATXN1 sponges miR-526b-3p in Ago2/RISC to increase MMP2/VEGFA and enhance angiogenesis. |
| Confidence score: |
0.7763 |