| Expression pattern: |
UP |
| Associated gene: |
TMTC3 |
| Associated microRNA: |
miR-944 |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion, tube formation, angiogenic capacity, and tumor formation in vivo; functions as an oncogene in HCC development. |
| Molecular mechanism: |
circMETTL15 acts as a competing endogenous RNA by binding miR-944, thereby regulating TMTC3 expression and promoting HCC malignancy through the miR-944/TMTC3 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Potential target and biomarker for HCC treatment. |
| Description: |
circMETTL15 is up-regulated in HCC tissues and HCC cell lines and is mainly localized in the cytoplasm. It promotes HCC cell proliferation, migration, invasion, angiogenic tube formation, and in vivo tumor growth by acting as a ceRNA that binds miR-944 and relieves repression of TMTC3. The study suggests circMETTL15 may serve as a therapeutic target or biomarker for HCC treatment. |
| Confidence score: |
0.8175 |