circRNA basic information
circBase ID: hsa_circ_0089552
Name: hsa_circ_NOTCH1
Synonym: circNOTCH1
Host Gene: NOTCH1
Genomic location(hg19): chr9:139402406-139418431:-
Genomic location(hg38): chr9:136507954-136523979:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small‐cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

A549; H1299
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: METTL14, NOTCH1 mRNA, YAP1, TEAD, QKI
Associated microRNA: -
Biological function: promotes cell growth; promotes tumour growth; increases NOTCH1 expression
Molecular mechanism: circNOTCH1 competes with NOTCH1 mRNA for METTL14 binding, reducing m6A modification on NOTCH1 mRNA and increasing NOTCH1 mRNA stability/translation; GPER induces circNOTCH1 biogenesis via YAP1-TEAD transcriptional activation of QKI
Biological pathway or process:

proliferation (promotes); m6A modification (other); mRNA stability (promotes); Notch (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; FISH / smFISH; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Western Blot; Transfection; MTT; Colony Formation Assay; In Vivo Animal Model
Clinical significance:

-
Description:

In NSCLC cells, circNOTCH1 is induced downstream of GPER via YAP1-TEAD-mediated transcriptional upregulation of the circRNA biogenesis factor QKI. circNOTCH1 promotes NSCLC cell growth and xenograft tumour growth by competing with NOTCH1 mRNA for METTL14 binding, thereby altering m6A-related regulation and increasing NOTCH1 mRNA stability and NOTCH1 protein expression.
Confidence score:

0.6996
Other information
Title:

G protein-coupled oestrogen receptor promotes cell growth of non-small cell lung cancer cells via YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 signalling.

Journal: Journal of cellular and molecular medicine
Published: 2021
PubMed ID: 33237585
Study type:

combined biological and clinical study
Data availability: The data sets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -