circRNA basic information
circBase ID: -
Name: hsa_circ_SLC7A6
Synonym: circ-SLC7A6
Host Gene: SLC7A6
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; para-carcinoma tissues; precancerous normal tissues; adjacent normal tissues; tumor tissues; lungs

Cell lines:

A549; H460

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: PTEN, E-cadherin, p21, TIMP3, MAPK10, QKI
Associated microRNA: miR-21
Biological function: Acts as a tumor suppressor in NSCLC; inhibits NSCLC cell proliferation, invasion, tumor growth and lung metastasis.
Molecular mechanism: circ-SLC7A6 functions as a cytoplasmic miR-21 sponge, suppressing miR-21 and elevating miR-21 downstream tumor suppressors including PTEN, E-cadherin, p21, TIMP3, MAPK10 and QKI; QKI binds introns flanking circ-SLC7A6 and facilitates circ-SLC7A6 production.
Biological pathway or process:

proliferation (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); other pathway/process (other)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

Low circ-SLC7A6 was associated with larger tumor size, lymph node metastasis, advanced clinical stage and adverse outcome; patients with high circ-SLC7A6 had longer overall and disease-free survival time, implying prognostic indicator potential in NSCLC.

Description:

circ-SLC7A6 is downregulated in NSCLC and low expression is associated with aggressive clinicopathological features and worse survival. Functionally, circ-SLC7A6 suppresses NSCLC proliferation, invasion, tumor growth and lung metastasis by sponging oncogenic miR-21 and increasing multiple miR-21 downstream tumor suppressors. QKI is also implicated in a positive regulatory loop by binding introns flanking circ-SLC7A6 and facilitating its production.

Confidence score:

0.8769

Other information
Title:

Circular RNA circ-SLC7A6 acts as a tumor suppressor in non-small cell lung cancer through abundantly sponging miR-21.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2020
PubMed ID: 32794418
Study type:

combined biological and clinical study

Data availability: GSE101684; GSE112214; GSE101586; available from the corresponding author on reasonable request
Code availability: -