circRNA basic information
circBase ID: hsa_circ_0004140
Name: hsa_circ_YAP1
Synonym: circ-LECRC
Host Gene: YAP1
Genomic location(hg19): chr11:102056748-102080295:+
Genomic location(hg38): chr11:102186017-102209564:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; matched adjacent non-cancer tissues; adjacent non-tumour tissues; lung
Cell lines:

SW480; SW620; RKO; DLD-1; HCT116; HT-29; 293T; FHC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: KLF4, YAP1 pathway, EGFR, MYC, BIRC5, CTGF, AGO2
Associated microRNA: miR-135b-5p
Biological function: Circ-LECRC suppresses CRC cell proliferation, migration, invasion, tumorigenicity and lung metastasis, and promotes apoptosis.
Molecular mechanism: Circ-LECRC functions as a ceRNA sponge for miR-135b-5p, upregulates KLF4, and inhibits hyperactivation of oncogenic YAP1 signalling and YAP1 downstream genes including EGFR, MYC, BIRC5, and CTGF.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); ceRNA regulation (promotes); Hippo/YAP (inhibits); mRNA stability (promotes)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Co-IP; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ-LECRC expression correlated with lower TNM stage and favourable survival prognosis, while low expression correlated with more aggressive disease and poor prognosis.
Description:

Circ-LECRC is a YAP1-derived circRNA that is downregulated in colorectal cancer and acts as a tumor suppressor. It sponges miR-135b-5p to increase KLF4 expression, thereby inhibiting oncogenic YAP1 signalling and reducing proliferation, migration, invasion, tumorigenicity, and lung metastasis while promoting apoptosis. Clinically, higher circ-LECRC expression is associated with lower TNM stage and better survival prognosis.
Confidence score:

0.887
Other information
Title:

YAP derived circ-LECRC functions as a "brake signal" to suppress hyperactivation of oncogenic YAP signalling in colorectal cancer.

Journal: Cancer letters
Published: 2022
PubMed ID: 35131381
Study type:

combined biological and clinical study
Data availability: GSE21510; TCGA; Supplementary data https://doi.org/10.1016/j.canlet.2022.215589
Code availability: -