| Expression pattern: |
UP |
| Associated gene: |
BRD4, MMP9, Ago2, Shh, Gli1 |
| Associated microRNA: |
miR-125a |
| Biological function: |
Promotes proliferation and migration in vitro; facilitates tumor growth and brain metastasis in vivo; induces apoptosis when silenced. |
| Molecular mechanism: |
Acts as a miR-125a sponge, upregulates BRD4, leading to increased MMP9 via SHH signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); metastasis (promotes); Hedgehog (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
Upregulated in BCBM tissues and plasma; higher expression in primary tumor associated with shorter BMFS; potential diagnostic and prognostic biomarker. |
| Description: |
circBCBM1 (hsa_circ_0001944), derived from the FIRRE locus, is upregulated in breast cancer brain metastasis cells, tissues, and patient plasma. It promotes proliferation, migration, tumor growth and brain metastasis by sponging miR-125a to upregulate BRD4, which increases MMP9 via SHH signaling. High circBCBM1 in primary tumors predicts shorter brain metastasis-free survival, supporting diagnostic/prognostic potential. |
| Confidence score: |
0.8817 |