| Expression pattern: |
UP |
| Associated gene: |
ZEB2 |
| Associated microRNA: |
miR-377 |
| Biological function: |
Promotes bladder cancer cell growth/proliferation, migration and invasion; arrests cell cycle at G0/G1 when silenced; promotes apoptosis when silenced. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-377, thereby promoting ZEB2 expression; ZEB2 restoration rescues the suppressive effects of circZFR knockdown. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
Higher circZFR levels were positively correlated with pathological T stage, grade, lymphatic metastasis, recurrence, and poorer progression-free survival (PFS) and overall survival (OS); ROC suggested diagnostic value (AUC = 0.8216). |
| Description: |
CircZFR is up-regulated in bladder cancer tissues and cell lines and is associated with worse PFS/OS. Functionally, circZFR promotes proliferation, migration and invasion and inhibits apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-377 to upregulate ZEB2, and ZEB2 restoration rescues the effects of circZFR knockdown. |
| Confidence score: |
0.7929 |