| Expression pattern: |
UP |
| Associated gene: |
PDK2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes HCC cell proliferation, colony formation, cell cycle progression, migration, invasion, metastasis and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
hsa_circ_0005397 functions as a ceRNA/miRNA sponge for miR-326, thereby positively regulating PDK2 expression and facilitating HCC progression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0005397 expression was associated with poor survival and with tumor size, TNM stages, lymph node metastasis and distant metastasis in HCC patients; the hsa_circ_0005397/miR-326/PDK2 axis might be a promising therapeutic strategy for HCC. |
| Description: |
hsa_circ_0005397 is up-regulated in HCC tissues and cell lines and high expression predicts poor survival. Functionally, it promotes HCC progression by enhancing proliferation, migration, invasion, metastasis and tumor growth while suppressing apoptosis. Mechanistically, hsa_circ_0005397 sponges miR-326 to up-regulate PDK2, forming the hsa_circ_0005397/miR-326/PDK2 axis. |
| Confidence score: |
0.8351 |