| Expression pattern: |
UP |
| Associated gene: |
YAP1, Ago2 |
| Associated microRNA: |
miR-498 |
| Biological function: |
promotes proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth and metastasis |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-498 to relieve repression of YAP1 and activate YAP1/Hippo signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); Hippo/YAP (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Sanger Sequencing; divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); RNA-seq; In Vivo Animal Model; H&E Staining; Survival Analysis; ROC Analysis |
| Clinical significance: |
High hsa_circ_0085576 expression was significantly correlated with tumor size, clinical stage, and metastasis status and poorer survival. |
| Description: |
hsa_circ_0085576 is up-regulated in ccRCC tissues and cell lines and mainly localizes to the cytoplasm. It promotes ccRCC proliferation, migration/invasion and metastasis while inhibiting apoptosis by sponging miR-498 and derepressing YAP1, activating Hippo/YAP1 signaling; high expression indicates poor prognosis. |
| Confidence score: |
0.8802 |