| Expression pattern: |
UP |
| Associated gene: |
PDK1, AKT, Ago2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
promotes NSCLC cell viability/growth, migration and invasion, suppresses apoptosis, and promotes tumor growth in vivo |
| Molecular mechanism: |
circPRKCA acts as a miRNA sponge for miR-330-5p to regulate PDK1 expression and AKT activity (ceRNA axis) |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High circPRKCA was correlated with TNM stage and lymph node metastasis of NSCLC patients. |
| Description: |
circPRKCA is up-regulated in NSCLC tissues and cell lines and acts oncogenically. It sponges miR-330-5p to increase PDK1 expression and activate AKT signaling, thereby promoting proliferation/viability, migration and invasion and inhibiting apoptosis; circPRKCA knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.7289 |