| Expression pattern: |
UP |
| Associated gene: |
YAP1, AGO2, LATS1/2, MST1 |
| Associated microRNA: |
miR-200a-3p |
| Biological function: |
promotes proliferation and migration; promotes cell cycle progression; promotes tumor growth in vivo |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge for miR-200a-3p to upregulate YAP1 and inactivate Hippo signaling |
| Biological pathway or process: |
Hippo/YAP (inhibits); proliferation (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation; RNA-seq |
| Clinical significance: |
High expression hsa_circ_0005273 was positively associated with TNM stage, lymph node metastasis, tumor size and distant metastasis; potential biomarker and therapeutic target |
| Description: |
hsa_circ_0005273 is up-regulated in breast cancer tissues and cell lines and promotes tumorigenesis (proliferation, migration, cell-cycle progression, and xenograft growth). Mechanistically, it acts as a cytoplasmic miR-200a-3p sponge to increase YAP1 and thereby inactivate Hippo signaling. |
| Confidence score: |
0.8771 |