| Expression pattern: |
UP |
| Associated gene: |
CDK8, Ago2 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
Promotes paclitaxel resistance, proliferation, migration, invasion and autophagy, and inhibits apoptosis in PTX-resistant breast cancer cells; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-1299 to upregulate CDK8 expression, thereby promoting PTX resistance and malignant phenotypes. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); autophagy (promotes); drug resistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0006528 is up-regulated in paclitaxel-resistant breast cancer tissues and cells and promotes PTX resistance and malignant behaviors. Mechanistically, it sponges miR-1299 to upregulate CDK8, thereby enhancing proliferation/migration/invasion and autophagy while suppressing apoptosis, and it promotes tumor growth in xenograft models. |
| Confidence score: |
0.7591 |