| Expression pattern: |
UP |
| Associated gene: |
TXNDC5 |
| Associated microRNA: |
miR-1296-5p |
| Biological function: |
Promotes HCC cell viability and colony formation, facilitates cell cycle progression, inhibits apoptosis, and promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA to sponge miR-1296-5p, thereby upregulating TXNDC5. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
High circ_0000517 expression is associated with worse TNM stage. |
| Description: |
circ_0000517 is up-regulated in HCC tissues and cell lines and mainly localizes to the cytoplasm. It promotes HCC progression by sponging miR-1296-5p to relieve repression of TXNDC5, thereby enhancing proliferation/colony formation and cell-cycle progression and inhibiting apoptosis, with supportive xenograft evidence. |
| Confidence score: |
0.7983 |