| Expression pattern: |
UP |
| Associated gene: |
HMGA2, Ago2 |
| Associated microRNA: |
miR-149 |
| Biological function: |
Promotes CRC cell proliferation and tumor growth; inhibits apoptosis; promotes migration and invasion; promotes in vivo tumor growth and lung metastasis. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-149, thereby relieving miR-149-mediated repression of HMGA2. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
May serve as an early diagnostic biomarker for detection of CRC; higher expression associated with metastasis and advanced stages. |
| Description: |
circRNA 100146 (hsa_circ_0011385, from EIF3I) is upregulated in colorectal cancer tissues and cell lines. It promotes CRC proliferation, migration/invasion and tumor growth/metastasis and inhibits apoptosis by sponging miR-149 to upregulate HMGA2 (circRNA 100146/miR-149/HMGA2 axis). |
| Confidence score: |
0.8275 |