circRNA basic information
circBase ID: hsa_circ_0018534
Name: hsa_circ_SLC25A16
Synonym: circSLC25A16
Host Gene: SLC25A16
Genomic location(hg19): chr10:70252888-70253327:-
Genomic location(hg38): chr10:68493131-68493570:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue; adjacent normal tissue; tumor tissue

Cell lines:

H1299; A549; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CISD2
Associated microRNA: miR-335-5p
Biological function: Promotes NSCLC cell proliferation, migration and glycolysis, and inhibits apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Acts as a miR-335-5p sponge to upregulate CISD2 (ceRNA mechanism).
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); glycolysis (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation

Clinical significance:

circSLC25A16 may serve as a novel biomarker for NSCLC treatment.

Description:

circSLC25A16 is up-regulated in NSCLC tissues and cell lines and promotes proliferation, migration and glycolysis while suppressing apoptosis. Mechanistically, it sponges miR-335-5p to increase CISD2 expression, thereby enhancing NSCLC progression and xenograft tumor growth.

Confidence score:

0.8172

Other information
Title:

CircSLC25A16 facilitates the development of non-small-cell lung cancer through the miR-335-5p/CISD2 axis.

Journal: Thoracic cancer
Published: 2024
PubMed ID: 38803052
Study type:

combined biological and clinical study

Data availability: -
Code availability: -