circRNA basic information
circBase ID: hsa_circ_0018534
Name: hsa_circ_SLC25A16
Synonym: -
Host Gene: SLC25A16
Genomic location(hg19): chr10:70252888-70253327:-
Genomic location(hg38): chr10:68493131-68493570:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small-cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue samples; adjacent non-tumor tissues

Cell lines:

H460; H1299; A549; NHBE; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: HIF-1alpha, LDHA
Associated microRNA: miR-488-3p
Biological function: accelerated glycolysis and promoted proliferation; promoted in vivo tumor growth
Molecular mechanism: ceRNA mechanism: circSLC25A16 sponges miR-488-3p to relieve repression of HIF-1alpha, which transcriptionally activates LDHA
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis

Clinical significance:

high-expression of circSLC25A16 was associated with unfavorable prognosis

Description:

circSLC25A16 is up-regulated in NSCLC and promotes glycolysis and proliferation. It functions mainly in the cytoplasm as a miRNA sponge for miR-488-3p, thereby increasing HIF-1alpha and enhancing LDHA transcription, which supports tumor growth and is associated with poor prognosis.

Confidence score:

0.8701

Other information
Title:

Circular RNA circSLC25A16 contributes to the glycolysis of non-small-cell lung cancer through epigenetic modification.

Journal: Cell death & disease
Published: 2020
PubMed ID: 32513983
Study type:

combined biological and clinical study

Data availability: Supplementary Information accompanies this paper at (10.1038/s41419-020-2635-5).
Code availability: -