circRNA basic information
circBase ID: hsa_circ_0069982
Name: hsa_circ_MTHFD2L
Synonym: CircMTHFD2L
Host Gene: MTHFD2L
Genomic location(hg19): chr4:75040222-75091111:+
Genomic location(hg38): chr4:74174505-74225394:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissue; normal adjacent mucosal tissues
Cell lines:

HGC-27; MGC-803; MKN-28; MKN-45; AGS; SGC-7901; GES-1; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: -
Biological function: Not specified (circRNA RNA itself was not the functional effector; its encoded protein CM-248aa mediates tumor-suppressive effects).
Molecular mechanism: Encodes CM-248aa; the circRNA RNA sequence itself is insufficient to suppress malignant phenotypes when translation is blocked.
Biological pathway or process:

not specified
Detected method:
Q
B
S
Validation methods:

RNA-seq; RT-qPCR; Northern Blot; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation
Clinical significance:

circMTHFD2L is downregulated in GC tissues and GC cell lines compared with controls.
Description:

circMTHFD2L (hsa_circ_0069982) is downregulated in gastric cancer and mainly localized in the cytoplasm. Its biological impact in this study is primarily through encoding the peptide CM-248aa; restoring circMTHFD2L RNA without CM-248aa translation does not reverse malignant phenotypes.
Confidence score:

0.6334
Other information
Title:

Circular MTHFD2L RNA-encoded CM-248aa inhibits gastric cancer progression by targeting the SET-PP2A interaction.

Journal: Molecular therapy : the journal of the American Society of Gene Therapy
Published: 2023
PubMed ID: 37101395
Study type:

combined biological and clinical study
Data availability: The RNA sequencing data of the present study are available from the corresponding author upon reasonable request.; https://doi.org/10.1016/j.ymthe.2023.04.013
Code availability: -