circRNA basic information
circBase ID: hsa_circ_0000566
Name: hsa_circ_VRK1
Synonym: circVRK1
Host Gene: VRK1
Genomic location(hg19): chr14:97299803-97327072:+
Genomic location(hg38): chr14:96833466-96860735:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 1060198
MONDO name: ischemic stroke
Disease details: ischemic stroke / IS
Disease DO ID:
 -
Disease MeSH ID:
 -
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HBMECs; HUVECs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MLLT1
Associated microRNA: miR-150-5p
Biological function: CircVRK1 knockdown alleviates OGD-induced HBMEC injury by promoting migration and angiogenesis, repressing cell death, and reducing inflammatory response and oxidative stress.
Molecular mechanism: CircVRK1 acts as a ceRNA by sponging miR-150-5p, thereby positively regulating MLLT1 expression in OGD-induced HBMEC injury.
Biological pathway or process:

ceRNA regulation (promotes); migration (inhibits); angiogenesis (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Bioinformatics Analysis; Transfection; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down
Clinical significance:

CircVRK1 might provide a promising curative target for IS patients.
Description:

CircVRK1 is upregulated in OGD-treated HBMECs, an in vitro model of ischemic stroke. Its knockdown protects HBMECs from OGD-induced injury by reducing cell death, inflammation and oxidative stress while improving migration and angiogenesis, mediated through the miR-150-5p/MLLT1 ceRNA axis.
Confidence score:

0.7624
Other information
Title:

Circvrk1 downregulation attenuates brain microvascular endothelial cell damage induced by oxygen-glucose deprivation through modulating the miR-150-5p/MLLT1 axis.

Journal: Experimental brain research
Published: 2023
PubMed ID: 36735043
Study type:

biological research
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -