| Expression pattern: |
UP |
| Associated gene: |
CBX4, VEGFA |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
promotes RCC cell proliferation, migration, invasion and in vivo tumor growth and lung metastasis; promotes EMT |
| Molecular mechanism: |
ceRNA/miRNA sponge: circTLK1 sponges miR-136-5p to increase CBX4 expression; CBX4 increases VEGFA |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circTLK1 expression is positively correlated with distant metastasis and unfavorable prognosis; high circTLK1 expression is associated with lower overall survival and lower disease-free survival |
| Description: |
circTLK1 (hsa_circ_0004442), derived from TLK1, is up-regulated in renal cell carcinoma and mainly localized in the cytoplasm. It promotes RCC proliferation, EMT-associated migration/invasion and in vivo tumor growth/metastasis by acting as a ceRNA that sponges miR-136-5p, thereby increasing CBX4 and downstream VEGFA. High circTLK1 expression is associated with distant metastasis and poorer OS/DFS, suggesting prognostic/diagnostic potential. |
| Confidence score: |
0.8793 |