| Expression pattern: |
UP |
| Associated gene: |
TPD52, NF-kappaB p65, p-NF-kappaB p65 |
| Associated microRNA: |
miR-495-3p |
| Biological function: |
Promotes activation of human lung fibroblasts, enhances MRC-5 cell migration and invasion, increases fibroblast activation markers and pro-inflammatory cytokines. |
| Molecular mechanism: |
Exosomal circEZH2 functions as a ceRNA to regulate miR-495-3p, increases TPD52 expression, and activates the NF-kappaB pathway to promote fibroblast activation. |
| Biological pathway or process: |
NF-kappaB (promotes); ceRNA regulation (promotes); migration (promotes); invasion (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
circEZH2 is upregulated in serum exosomes from non-small cell lung cancer patients, suggesting potential relevance as an exosomal disease-associated circRNA. |
| Description: |
NSCLC-derived exosomal hsa_circ_0006357 (circEZH2) is upregulated in patient serum exosomes and in MRC-5 cells exposed to NSCLC-cell exosomes. circEZH2 promotes fibroblast activation, migration, invasion, and inflammatory gene expression by acting through the miR-495-3p/TPD52 axis and activating the NF-kappaB pathway. |
| Confidence score: |
0.6815 |