circRNA basic information
circBase ID: hsa_circ_0109177
Name: hsa_circ_NOTCH3
Synonym: circ_NOTCH3
Host Gene: NOTCH3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004984
MONDO name: basal-like breast carcinoma
Disease details: basal-like breast carcinoma
Disease DO ID:
 -
Disease MeSH ID:
 -
Disease NCIt ID:
 C53558
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BLBC tumor tissue; paired normal breast tissues
Cell lines:

MDA-MB-468; MCF-10A; HEK293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: KLF12
Associated microRNA: miR-205-5p
Biological function: promotes proliferation, migration and invasion; inhibits apoptosis
Molecular mechanism: circ_NOTCH3 acts as a miRNA sponge for miR-205-5p to upregulate KLF12.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

circ_NOTCH3 could be a powerful biomarker for BLBC.
Description:

circ_NOTCH3 is up-regulated in BLBC tissues and cells and promotes malignant phenotypes (proliferation, migration, invasion) while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-205-5p, thereby relieving repression of KLF12 and promoting BLBC progression.
Confidence score:

0.6815
Other information
Title:

circ_NOTCH3 Functions as a Protooncogene Competing With miR-205-5p, Modulating KLF12 Expression and Promoting the Development and Progression of Basal-Like Breast Carcinoma.

Journal: Frontiers in oncology
Published: 2020
PubMed ID: 33552974
Study type:

combined biological and clinical study
Data availability: PRJNA663412
Code availability: -